Ultrasensitive and high efficiency screen of de novo low
May 22, 20170183;32;Screening de novo low frequency mutations in HCC tumours. The frequency of these mutations ranged from 0.0028 to 0.087 and included different mutation types ( Fig. 5a,b ). To validate these low frequency mutations, we designed ultra deep amplicon sequencing (from 30,000 215; to 250,000 215; ) for each candidate.